DNA sequence of the transfer RNA region of bacteriophage T4: implications for transfer RNA synthesis.
نویسندگان
چکیده
منابع مشابه
The nucleotide sequence of threonine transfer RNA coded by bacteriophage T4.
The nucleotide sequence of a low molecular weight RNA coded by bacteriophage T4 (and previously identified as species alpha) has been determined. The molecule is of particular biological interest for its associated biosynthetic properties. This RNA is 76 nucleotides in length, contains eight modified bases, and can be arranged in a cloverleaf configuration common to tRNAs. The anticodon sequenc...
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The nucleotide sequence of a T4 tRNA with an anticodon for glycine has been determined using (32)P-labeled material from T4-infected cultures of Escherichiacoli. The sequence is: pGCGGAUAUCGUAUAAUGmGDAUUACCUCAGACUUCCAApsiCUGAUGAUGUGAGTpsiCGAUUCUCAUUAUCCGCUCCA-OH. The 74 nucleotide sequence can be arranged in the classic cloverleaf pattern for tRNAs. The anticodon of T4 tRNA(Gly) is UCC with a p...
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A U-G-A suppressor of bacteriophage T4, designated psu4+op, has been isolated and characterized. The transfer RNA species previously shown to have an anticodon sequence complementary to arginine codons is affected by the psu4+op mutation. Wild type and psu4+op arginine tRNAs have the same sequence except for their anticodons, where U-C-U in the wild type species is mutated to U-C-A in the psu4+...
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Transfer RNA's were isolated from Euglena gracilis. Chloroplast cistrons for tRNA were quantitated by hybridizing tRNA to ct DNA. Species of tRNA hybridizing to ct DNA were partially purified by hybridization-chromatography. The tRNA's hybridizing to ct DNA and nuclear DNA appear to be different. Total cellular tRNA was hybridized to ct DNA to an equivalent of approximately 25 cistrons. The tot...
متن کاملIn Vitro Synthesis of Transfer RNA
We have described an in vitro system in which active s&r tRNATyr is synthesized from a @Ops&r DNA template. Using this system, we have identified four essential components that are required for synthesis of tRNA. The first of these is DNA-dependent RNA polymerase. It has been shown that a crude preparation of DNA-dependent RNA polymerase synthesizes s&r tRNATyr precursor similar to that which h...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1981
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.78.2.889